The GTF3C6 Antibody (CAB13233) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and exhibits high specificity and sensitivity towards human samples, making it ideal for Western blot applications. By binding to the GTF3C6 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential component in studies related to transcriptional regulation and gene expression.GTF3C6, also known as General Transcription Factor IIIC Subunit 6, is crucial for the initiation of transcription by RNA polymerase III.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
GTF3C6 Antibody
SKU:
CAB13233
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
C6orf51, TFIIIC35, bA397G5.3, GTF3C6
Positive Sample:
HeLa
Cellular Localization:
Nucleus.
Calculated MW:
24kDa
Observed MW:
24kDa
RNA polymerases are unable to initiate RNA synthesis in the absence of additional proteins called general transcription factors (GTFs). GTFs assemble in a complex on the DNA promoter and recruit the RNA polymerase. GTF3C family proteins (e.g., GTF3C1, MIM 603246) are essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA (MIM 180420), tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin.
Purification Method
Affinity purification
Gene ID
112495
RRID
AB_2760086
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using GTF3C6 Rabbit pAb (CAB13233) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
