The HAS3 Antibody (CAB13498) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it an ideal choice for various experimental techniques, including Western blotting.HAS3, also known as hyaluronan synthase 3, plays a crucial role in maintaining tissue hydration and structural integrity, making it essential for processes such as wound healing, tissue repair, and inflammation. Dysregulation of HAS3 expression has been implicated in various pathological conditions, including cancer metastasis, fibrosis, and inflammatory diseases.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HAS3 Antibody
SKU:
CAB13498
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HAS3
Positive Sample:
PC-3, Rat lung
Cellular Localization:
Membrane, Multi-Pass Membrane Protein.
Calculated MW:
63kDa
Observed MW:
63kDa
The protein encoded by this gene is involved in the synthesis of the unbranched glycosaminoglycan hyaluronan, or hyaluronic acid, which is a major constituent of the extracellular matrix. This gene is a member of the NODC/HAS gene family. Compared to the proteins encoded by other members of this gene family, this protein appears to be more of a regulator of hyaluronan synthesis. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
3038
RRID
AB_2760361
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HAS3 Rabbit pAb (CAB13498) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of A549 cells using HAS3 Rabbit pAb (CAB13498). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
