The HCRTR1 Antibody (CAB14740) is a high-quality antibody developed for reliable detection and analysis of target proteins. This cell surface receptor is involved in the regulation of sleep and wakefulness, making it a key target for studies on sleep disorders and related conditions.Raised in rabbits, this antibody is highly specific to human samples and has been validated for use in Western blot applications. By binding to the HCRTR1 protein, researchers can accurately detect and analyze its expression in various cell types, providing valuable insights into the mechanisms underlying sleep regulation.The HCRTR1 antibody is particularly valuable for investigating the role of hypocretin signaling in sleep disorders such as narcolepsy, insomnia, and circadian rhythm disruptions.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HCRTR1 Antibody
SKU:
CAB14740
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
HPLL FKST ARRA RGSI LGIW AVSL AIMV PQAA VMEC SSVL PELA NRTR LFSV CDER WADD LYPK IYHS CFFI VTYL APLG LMAM AYFQ IFRK LWGR QIPG T
Tested Applications:
WBELISAIF-P
Recommended Dilution:
WB
1:500 - 1:1000
IF-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
OX1R, OXR1, ORXR1, HCRTR1
Positive Sample:
C6
Cellular Localization:
Cell Membrane, Multi-Pass Membrane Protein.
Calculated MW:
48kDa
Observed MW:
48kDa
The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein selectively binds the hypothalamic neuropeptide orexin A. A related gene (HCRTR2) encodes a G-protein coupled receptor that binds orexin A and orexin B.
Purification Method
Affinity purification
Gene ID
3061
RRID
AB_2761618
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells, using HCRTR1 Rabbit pAb (CAB14740) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of paraffin-embedded mouse brain using HCRTR1 Rabbit pAb (CAB14740) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
