The HDGFRP2 Antibody (CAB7484) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to human samples and is suitable for use in Western blot, immunofluorescence, and immunohistochemistry applications. By targeting the HDGFRP2 protein, this antibody enables the accurate detection and analysis of HDGFRP2 expression in a variety of cell types.HDGFRP2, also known as Hepatoma-Derived Growth Factor Related Protein 2, is a key player in regulating cell growth and differentiation processes.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
HDGFRP2 Antibody
SKU:
CAB7484
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HRP2, HDGF2, HRP-2, HDGF-2, HDGFRP2
Positive Sample:
Mouse testis
Cellular Localization:
Nucleus.
Calculated MW:
74kDa
Observed MW:
110kDa
This gene encodes a member of the hepatoma-derived growth factor (HDGF) family. The protein includes an N-terminal PWWP domain that binds to methyl-lysine-containing histones, with specific binding of this protein to tri-methylated lysines 36 and 79 of histone H3, and di- and tri-methylated lysine 20 of histone H4. The protein functions in LEDGF/p75-independent HIV-1 replication by determining HIV-1 integration site selection. Alternative splicing of this gene results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
84717
RRID
AB_2768015
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from mouse testis, using HDGFRP2 Rabbit pAb (CAB7484) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of A549 cells using HDGFRP2 Rabbit pAb (CAB7484).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution.
