The HEATR1 Antibody (CAB13102) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reacts strongly with samples from various species, making it a versatile option for a range of experimental settings.HEATR1 is a key player in RNA splicing and is essential for the proper functioning of the spliceosome, the molecular machine responsible for removing non-coding sequences from pre-mRNA. Dysregulation of RNA processing can lead to a variety of diseases, including cancer and genetic disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
HEATR1 Antibody
SKU:
CAB13102
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BAP28, UTP10, HEATR1
Positive Sample:
HeLa
Cellular Localization:
Nucleus, Nucleolus.
Calculated MW:
242kDa
Observed MW:
242kDa
Enables RNA binding activity. Involved in positive regulation of rRNA processing and positive regulation of transcription by RNA polymerase I. Located in fibrillar center and mitochondrion. Implicated in pancreatic ductal carcinoma. Biomarker of glioblastoma.
Purification Method
Affinity purification
Gene ID
55127
RRID
AB_2759951
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using HEATR1 Rabbit pAb (CAB13102) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
