The HEC1/NDC80 Monoclonal Antibody (CAB2392) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. RRID AB_2862998 Gene ID 10403 Swiss Prot Synonym HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80; HEC1/NDC80
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
HEC1/NDC80 Monoclonal Antibody
SKU:
CAB2392
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC0741
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. RRID AB_2862998 Gene ID 10403 Swiss Prot Synonym HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80; HEC1/NDC80
Purification Method:
Affinity purification
Gene ID:
10403
RRID:
AB_2862998
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HEC1/NDC80 Rabbit mAb (CAB2392) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3 s.
