The HEL308 Antibody (CAB12661) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, shows high specificity and sensitivity towards HEL308 in human samples and is suitable for use in Western blot applications. By binding to the HEL308 protein, this antibody allows for the detection and analysis of HEL308 in various cell types, making it an essential component for studies in DNA repair mechanisms and genome maintenance.HEL308 is a key player in DNA damage response pathways and is known for its ability to unwind DNA structures during the repair process.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HEL308 Antibody
SKU:
CAB12661
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HEL308
Positive Sample:
293T, LO2, HeLa, Raji, Mouse testis
Cellular Localization:
Nucleus.
Calculated MW:
124kDa
Observed MW:
124kDa
HEL308 is a single-stranded DNA-dependent ATPase and DNA helicase (Marini and Wood, 2002 [PubMed 11751861]).
Purification Method
Affinity purification
Gene ID
113510
RRID
AB_2759508
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using HEL308 Rabbit pAb (CAB12661) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
