The HER2/ErbB2 Polyclonal Antibody (CAB21768) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, has high reactivity with human samples and has been validated for use in applications such as Western blotting.HER2/ERBB2 is known to play a crucial role in cancer development, specifically in breast cancer where overexpression of this protein is associated with a more aggressive form of the disease. By using the HER2/ERBB2 Polyclonal Antibody, researchers can detect and analyze the expression of HER2/ERBB2 in various cell types, aiding in the understanding of its role in cancer progression.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
HER2/ErbB2 Polyclonal Antibody
SKU:
CAB21768
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Cell Membrane, Cytoplasm, Nucleus, Nucleus, Single-Pass Type I Membrane Protein, Perinuclear Region.
Calculated MW:
138kDa
Observed MW:
185kDa
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
Purification Method
Affinity purification
Gene ID
2064
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from MCF7 cells, using [KO Validated] HER2/ErbB2 Rabbit pAb (CAB21768) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Western blot analysis of lysates from wild type(WT) and HER2/ErbB2 Rabbit pAb knockout (KO) HeLa cells, using [KO Validated] HER2/ErbB2 Rabbit pAb (CAB21768) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
