The HEXIM1 Antibody (CAB5775) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has been validated for use in Western blot applications and is highly reactive with human samples. By specifically binding to Hexim1, researchers can detect and analyze the protein in various cell types, making it an ideal choice for studies in molecular biology and cancer research.Hexim1 is known for its role in controlling the activity of the positive transcription elongation factor b (P-TEFb), which is essential for the expression of specific genes involved in cell growth and development.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HEXIM1 Antibody
SKU:
CAB5775
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CLP1, EDG1, HIS1, MAQ1, HEXIM1
Positive Sample:
HeLa, 293F
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
41kDa
Observed MW:
66kDa
Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns.
Purification Method
Affinity purification
Gene ID
10614
RRID
AB_2766529
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using HEXIM1 Rabbit pAb (CAB5775) at 1:700 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using HEXIM1 Rabbit pAb (CAB5775) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using HEXIM1 Rabbit pAb (CAB5775) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using HEXIM1 Rabbit pAb (CAB5775) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
