Description
| Product Name: | Hexokinase(HK) Activity Assay Kit |
| Product Code: | MAES0242 |
| Sample Type: | Animal Tissue, Cell |
| Assay Time: | 30 min |
| Assay Type: | Enzyme Activity |
| Synonyms: | HK |
| Detection Instrument: | Microplate reader (440-450 nm, optimum wavelength: 450 nm) |
| Sensitivity: | 5.93 U/L |
| Detection Range: | 5.93-40.11 U/L |
| Inter-assay CV: | 0.091 |
| Intra-assay CV: | 0.033 |
HK converts glucose to glucose 6-phosphate, which is catalyzed by the enzyme. Meanwhile, NAD+ is reduced to NADH, which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of HK can be calculated by measuring the change of absorbance value at 450 nm.
| Storage: | -20°C |
| Valid Period: | 12 months |
| Other Instruments Required: | Centrifuge, 37℃ incubator |
