The HGFAC Antibody (CAB10114) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blotting applications.HGFA, also known as hepatocyte growth factor activator, plays a crucial role in the activation of hepatocyte growth factor (HGF), a key regulator of cell growth, motility, and morphogenesis. Dysregulation of the HGFA-HGF axis has been implicated in various diseases, including cancer, fibrosis, and thrombosis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HGFAC Antibody
SKU:
CAB10114
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HGFA, HGFAC
Positive Sample:
LO2, BT-474, SW620, Mouse liver, Mouse pancreas, Rat liver
Cellular Localization:
Secreted.
Calculated MW:
71kDa
Observed MW:
75kDa
This gene encodes a member of the peptidase S1 protein family. The encoded protein is first synthesized as an inactive single-chain precursor before being activated to a heterodimeric form by endoproteolytic processing. It acts as serine protease that converts hepatocyte growth factor to the active form. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
3083
RRID
AB_2757638
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HGFAC Rabbit pAb (CAB10114) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
