The HIBCH Antibody (CAB17129) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit polyclonal antibody is highly specific to human samples and is validated for use in Western blot applications.HIBCH plays a critical role in breaking down the amino acid valine, which is essential for cellular metabolism. Dysregulation of the valine degradation pathway has been linked to various metabolic disorders and diseases, making HIBCH an important target for study in fields such as metabolism, biochemistry, and genetics.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HIBCH Antibody
SKU:
CAB17129
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HIBYLCOAH, HIBCH
Positive Sample:
Mouse heart, Rat heart
Cellular Localization:
Mitochondrial Matrix, Mitochondrion.
Calculated MW:
43kDa
Observed MW:
38kDa/43kDa
This gene encodes the enzyme responsible for hydrolysis of both HIBYL-CoA and beta-hydroxypropionyl-CoA. Mutations in this gene have been associated with 3-hyroxyisobutyryl-CoA hydrolase deficiency. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
26275
RRID
AB_2769774
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using HIBCH Rabbit pAb (CAB17129) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunofluorescence analysis of L929 cells using HIBCH Rabbit pAb (CAB17129) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
