The HIF1beta/ARNT Monoclonal Antibody (CAB19532) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, has high specificity and sensitivity for detecting HIF1beta and ARNT proteins in human samples, making it ideal for use in Western blot applications.HIF1beta, also known as the beta subunit of Hypoxia-Inducible Factor 1, forms a heterodimer with ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator) to regulate the transcription of genes involved in oxygen homeostasis and response to low oxygen levels.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
HIF1beta/ARNT Monoclonal Antibody
SKU:
CAB19532
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC0010
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
405
RRID
AB_2862651
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using HIF1β/ARNT Rabbit mAb (CAB19532) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human colon using HIF1β/ARNT Rabbit mAb (CAB19532) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
