The HK3 Antibody (CAB8428) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity towards human samples and is specifically validated for use in Western blot applications. By binding to the hexokinase-3 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an ideal choice for studies in biochemistry and cancer research.Hexokinase-3 plays a crucial role in the glycolysis pathway, where it catalyzes the first step in glucose metabolism by converting glucose to glucose-6-phosphate.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HK3 Antibody
SKU:
CAB8428
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HXK3, HKIII, HK3
Positive Sample:
HepG2, HL-60, THP-1, NCI-H460, Mouse liver
Cellular Localization:
Cytosol, Extracellular Region, Mitochondrion.
Calculated MW:
99kDa
Observed MW:
110kDa
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate.
Purification Method
Affinity purification
Gene ID
3101
RRID
AB_2769796
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HK3 Rabbit pAb (CAB8428) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of A-549 cells using HK3 Rabbit pAb (CAB8428) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using HK3 Rabbit pAb (CAB8428) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using HK3 Rabbit pAb (CAB8428) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
