The HLX Antibody (CAB7436) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly reactive with human samples and is validated for use in various applications such as immunohistochemistry and flow cytometry.HLX is known for its role in regulating cell proliferation, differentiation, and apoptosis, making it a key player in developmental biology and cancer research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
HLX Antibody
SKU:
CAB7436
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HB24, HLX1, HLX
Positive Sample:
HL-60, Raji, THP-1, 293T
Cellular Localization:
Nucleus.
Calculated MW:
51kDa
Observed MW:
51kDa
Enables sequence-specific DNA binding activity. Predicted to be involved in cell differentiation and regulation of transcription by RNA polymerase II. Predicted to act upstream of or within several processes, including animal organ development; enteric nervous system development; and regulation of T-helper cell differentiation. Predicted to be located in nucleus. Predicted to be part of chromatin.
Purification Method
Affinity purification
Gene ID
3142
RRID
AB_2767964
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HLX Rabbit pAb (CAB7436) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
