The HMGA1 Antibody (CAB1635) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reactive in human samples, making it ideal for Western blot applications. By binding to HMGA1, this antibody facilitates the detection and analysis of the protein in a variety of cell types.HMGA1 is a key player in gene regulation and has been implicated in a wide range of diseases including cancer, diabetes, and obesity. Its ability to manipulate gene expression makes it a promising target for therapeutic interventions in these conditions.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
HMGA1 Antibody
SKU:
CAB1635
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HMG-R, HMGIY, HMGA1A, HMGA1
Positive Sample:
293T
Cellular Localization:
Chromosome, Nucleus.
Calculated MW:
12kDa
Observed MW:
18kDa
This gene encodes a chromatin-associated protein involved in the regulation of gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of AT-rich regions in double-stranded DNA. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been identified on multiple chromosomes.
Purification Method
Affinity purification
Gene ID
3159
RRID
AB_2763693
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293T cells, using HMGA1 Rabbit pAb (CAB1635) at 1:3000 dilution._Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human stomach using HMGA1 Rabbit pAb (CAB1635) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
