The Hmox1 Polyclonal Antibody (CAB23650) is a high-quality antibody developed for reliable detection and analysis of target proteins. Predicted to enable several functions, including heme binding activity; heme oxygenase (decyclizing) activity; and protein homodimerization activity. Acts upstream of or within several processes, including cellular response to cisplatin; cellular response to metal ion; and regulation of macroautophagy. Located in nucleus. Is expressed in several structures, including alimentary system; central nervous system; early conceptus; genitourinary system; and sensory organ. Used to study hemochromatosis and malaria. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); cerebrovascular disease (multiple); factor VIII deficiency; lung disease (multiple); and sickle cell anemia. Orthologous to human HMOX1 (heme oxygenase 1).
This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
Hmox1 Polyclonal Antibody
SKU:
CAB23650
Size:
100μL, 20μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISAIF-P
Recommended Dilution:
WB
1:500 - 1:1000
IF-P
1:50 - 1:200
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HO1, HO-1, Hmox, Hemox, Hsp32, D8Wsu38e, Hmox1
Positive Sample:
NIH/3T3 treated with arsenite, Mouse spleen, Rat spleen
Cellular Localization:
Endoplasmic Reticulum Membrane.
Calculated MW:
33KDa
Observed MW:
30kDa/35kDa
Predicted to enable several functions, including heme binding activity; heme oxygenase (decyclizing) activity; and protein homodimerization activity. Acts upstream of or within several processes, including cellular response to cisplatin; cellular response to metal ion; and regulation of macroautophagy. Located in nucleus. Is expressed in several structures, including alimentary system; central nervous system; early conceptus; genitourinary system; and sensory organ. Used to study hemochromatosis and malaria. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); cerebrovascular disease (multiple); factor VIII deficiency; lung disease (multiple); and sickle cell anemia. Orthologous to human HMOX1 (heme oxygenase 1).
Purification Method
Affinity purification
Gene ID
15368
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from NIH/3T3 cells using Hmox1 Rabbit pAb (CAB23650) at 1:1000 dilution. NIH/3T3 cells were treated with arsenite(50 μM) at 37℃ for 8 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of various lysates using Hmox1 Rabbit pAb (CAB23650) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of Rat spleen tissue using Hmox1 Rabbit pAb (CAB23650) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.
