Description
hns Antibody (PACO64777)
The H3K9me2 Polyclonal Antibody (PAC064777) is a valuable tool for researchers studying epigenetic modifications and their role in gene regulation. This antibody, raised in rabbits, exhibits high specificity and sensitivity in detecting H3K9me2, an important histone modification associated with transcriptional repression.The H3K9me2 antibody is validated for use in various applications, including Western blot, immunofluorescence, and chromatin immunoprecipitation (ChIP). It specifically binds to the H3K9me2 mark on histone proteins, allowing for precise and reliable analysis of its presence in different cell types and biological samples.
Epigenetic regulation, including histone modifications like H3K9me2, plays a crucial role in controlling gene expression patterns and cell fate decisions. Dysregulation of these modifications has been linked to numerous diseases, including cancer, developmental disorders, and neurodegenerative conditions. By investigating the function and regulation of H3K9me2, researchers can gain insights into the underlying mechanisms of these diseases and potentially identify novel therapeutic targets.
| Product Name: | hns Antibody |
| Product Code: | PACO64777 |
| Size: | 50µL |
| Target Names: | hns |
| Species Reactivity: | Escherichia coli |
| Host Species: | Rabbit |
| Antigen Species: | Escherichia coli(strain K12) |
| Tested Applications: | ELISA, WB; Recommended dilution: WB:1:1000-1:5000 |
| Isotype: | IgG |
| Clone ID: | N/A |
| Conjugate: | Non-conjugated |
| Clonality: | Polyclonal |
| Antigen: | Recombinant Escherichia coli DNA-binding protein H-NS protein (2-137aa) |
| Form: | Liquid |
| Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Storage: | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Aliases: | DNA-binding protein H-NS (Heat-stable nucleoid-structuring protein) (Histone-like protein HLP-II) (Protein B1) (Protein H1), hns, bglY cur drdX hnsA msyA osmZ pilG topS |
| Uniprot ID: | P0ACF8 |
| Background: | A DNA-binding protein implicated in transcriptional repression (silencing) (PubMed:333393, PubMed:2128918, PubMed:8890170, PubMed:8913298, PubMed:9398522, PubMed:16963779, PubMed:17046956, PubMed:23543115). Also involved in bacterial chromosome organization and compaction (PubMed:6379600, PubMed:10982869, PubMed:21903814). H-NS binds tightly to AT-rich dsDNA and inhibits transcription (PubMed:2512122, PubMed:16963779, PubMed:17435766, PubMed:17881364, PubMed:23543115). Binds upstream and downstream of initiating RNA polymerase, trapping it in a loop and preventing transcription (PubMed:11714691). Binds to hundreds of sites, approximately half its binding sites are in non-coding DNA, which only accounts for about 10% of the genome (PubMed:16963779, PubMed:17046956, PubMed:23543115). Many of these loci were horizontally transferred (HTG); this offers the selective advantage of silencing foreign DNA while keeping it in the genome in case of need (PubMed:17046956, PubMed:17881364, PubMed:26789284). Suppresses transcription at many intragenic sites as well as transcription of spurious, non-coding RNAs genome-wide (PubMed:24449106). Repression of HTG by H-NS is thought to allow their DNA to evolve faster than non-H-NS-bound regions, and facilitates integration of HTG into transcriptional regulatory networks (PubMed:26789284). A subset of H-NS/StpA-regulated genes also require Hha (and/or Cnu, ydgT) for repression; Hha and Cnu increase the number of genes DNA bound by H-NS/StpA and may also modulate the oligomerization of the H-NS/StpA-complex (PubMed:23543115). The protein forms 2 clusters in the nucleoid which gather hns-bound loci together, bridging non-contiguous DNA, and causes DNA substantial condensation (PubMed:21903814). Binds DNA better at low temperatures than at 37 degrees Celsius; AT-rich sites nucleate H-NS binding, further DNA-binding is cooperative and this cooperativity decreases with rising temperature (PubMed:17435766, PubMed:17881364). Transcriptional repression can be inhibited by dominant-negative mutants of StpA or itself (PubMed:8755860). May effect transcriptional elongation (PubMed:25638302). Can increase translational efficiency of mRNA with suboptimal Shine-Dalgarno sequences (PubMed:20595230). Plays a role in the thermal control of pili and adhesive curli fimbriae production, by inducing transcription of csgD (PubMed:17010156). Plays a role in flagellar function (PubMed:11031114). Represses the CRISPR-cas promoters, permits only weak transcription of the crRNA precursor; its repression is antagonized by LeuO (PubMed:20132443, PubMed:20659289). Binds preferentially to the upstream region of its own gene recognizing two segments of DNA on both sides of a bend centered around -150 (PubMed:7934818). Overexpression suppresses secY24, a temperature-sensitive mutation (PubMed:1537791). Has also been reported to activate transcription of some genes (PubMed:4566454, PubMed:338303, PubMed:2128918). |
| Research Area: | Others |
