The HOXA11 Antibody (CAB2976) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. It specifically binds to the HOXA11 protein, allowing for accurate detection and analysis in a variety of cell types.HOXA11 plays a crucial role in the regulation of gene expression during development, making it a focus of interest in studies related to embryogenesis, tissue regeneration, and cancer biology.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HOXA11 Antibody
SKU:
CAB2976
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HOX1, HOX1I, RUSAT1, HOXA11
Positive Sample:
Mouse colon, Rat uterus, Rat kidney
Cellular Localization:
Nucleus.
Calculated MW:
34kDa
Observed MW:
30kDa
In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. This gene is involved in the regulation of uterine development and is required for female fertility. Mutations in this gene can cause radio-ulnar synostosis with amegakaryocytic thrombocytopenia.
Purification Method
Affinity purification
Gene ID
3207
RRID
AB_2764788
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using HOXA11 Rabbit pAb (CAB2976) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunofluorescence analysis of HeLa cells using HOXA11 Rabbit pAb (CAB2976) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
