The HOXB5 Antibody (CAB8123) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, has high specificity for human samples and has been validated for use in Western blot applications. By binding to the HOXB5 protein, this antibody allows for the detection and analysis of HOXB5 expression in various cell types, making it ideal for studies in developmental biology and cancer research.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HOXB5 Antibody
SKU:
CAB8123
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HOX2, HU-1, HOX2A, Hox2.1, HHO.C10, HOXB5
Positive Sample:
293F, Rat lung
Cellular Localization:
Nucleus.
Calculated MW:
29kDa
Observed MW:
29kDa
This gene is a member of the Antp homeobox family and encodes a nuclear protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox B genes located on chromosome 17. The encoded protein functions as a sequence-specific transcription factor that is involved in lung and gut development. Increased expression of this gene is associated with a distinct biologic subset of acute myeloid leukemia (AML) and the occurrence of bronchopulmonary sequestration (BPS) and congenital cystic adenomatoid malformation (CCAM) tissue.
Purification Method
Affinity purification
Gene ID
3215
RRID
AB_2769832
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat lung, using HOXB5 Rabbit pAb (CAB8123) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 0.2s.
Western blot analysis of various lysates using HOXB5 Rabbit pAb (CAB8123) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
