The HP1 gamma/CBX3 Monoclonal Antibody (CAB4283) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot and immunofluorescence applications.HP1 Gamma, a member of the heterochromatin protein 1 family, is involved in diverse cellular processes such as transcriptional regulation, DNA repair, and genome stability. Dysregulation of HP1 Gamma has been linked to various diseases including cancer, neurodegenerative disorders, and developmental abnormalities.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HP1 gamma/CBX3 Monoclonal Antibody
SKU:
CAB4283
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0952
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HECH, HP1-GAMMA, HP1Hs-gamma, HP1 gamma/CBX3
Positive Sample:
HeLa, SH-SY5Y, Mouse spleen, Mouse thymus, Rat brain
Cellular Localization:
Nucleus.
Calculated MW:
21kDa
Observed MW:
22kDa
At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.
Purification Method
Affinity purification
Gene ID
11335
RRID
AB_2863224
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HP1 gamma/CBX3 Rabbit mAb (CAB4283) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of lysates from Rat brain, using HP1 gamma/CBX3 Rabbit mAb (CAB4283) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
