The HS1BP3 Antibody (CAB8250) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, shows high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to HS1BP3, the antibody enables precise detection and analysis of this protein in a variety of cell types.HS1BP3, also known as hematopoietic cell-specific Lyn substrate 1 (HS1) binding protein 3, is involved in regulating cytoskeletal dynamics and membrane trafficking within cells. Its functions are crucial for processes like cell migration, adhesion, and signaling, making it a key player in cellular physiology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HS1BP3 Antibody
SKU:
CAB8250
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ETM2, HS1-BP3, HS1BP3
Positive Sample:
HepG2, Mouse liver, Mouse kidney, Mouse heart, Rat liver
Cellular Localization:
Endoplasmic Reticulum, Mitochondrion.
Calculated MW:
43kDa
Observed MW:
55kDa
The protein encoded by this gene shares similarity with mouse Hs1bp3, an Hcls1/Hs1-interacting protein that may be involved in lymphocyte activation.
Purification Method
Affinity purification
Gene ID
64342
RRID
AB_2769871
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HS1BP3 Rabbit pAb (CAB8250) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 15s.