The HSD17B3 Antibody (CAB7687) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated from rabbit immunizations, specifically targets HSD17B3 in human samples and is validated for use in Western blot assays.HSD17B3 is a key enzyme in the conversion of estrone to estradiol, a process involved in hormone production and regulation. Dysregulation of HSD17B3 activity has been implicated in various hormone-related disorders, including hormone-dependent cancers and metabolic diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HSD17B3 Antibody
SKU:
CAB7687
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EDH17B3, SDR12C2, HSD17B3
Positive Sample:
293T transfected with HSD17B3
Cellular Localization:
Endoplasmic Reticulum.
Calculated MW:
35kDa
Observed MW:
33kDa
This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia.
Purification Method
Affinity purification
Gene ID
3293
RRID
AB_2769877
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with HSD17B3 using HSD17B3 Rabbit pAb (CAB7687) at 1:2500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
