The HSD3B1 Monoclonal Antibody (CAB19266) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through immunization of rabbits, exhibits high specificity and sensitivity towards human samples, making it suitable for use in Western blotting and immunohistochemistry applications.HSD3B1, also known as 3-beta-Hydroxysteroid Dehydrogenase Type 1, plays a crucial role in steroid hormone production and has been implicated in various hormone-related conditions such as adrenal hyperplasia and hormone-dependent cancers.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
HSD3B1 Monoclonal Antibody
SKU:
CAB19266
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Clone Number:
ARC2437
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
The protein encoded by this gene is an enzyme that catalyzes the oxidative conversion of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, which leads to the production of all classes of steroid hormones. The encoded protein also catalyzes the interconversion of 3-beta-hydroxy- and 3-keto-5-alpha-androstane steroids.
Purification Method
Affinity purification
Gene ID
3283
RRID
AB_3076608
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HSD3B1 Rabbit mAb (CAB19266) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Rat adrenal gland tissue using HSD3B1 Rabbit mAb (CAB19266) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
