Human 2',5'-Oligoadenylate Synthetase 2 (OAS2) ELISA Kit
The Human 2'5'-Oligoadenylate Synthetase 2 (OAS2) ELISA Kit is a cutting-edge tool developed for the precise quantification of OAS2 levels in human samples including serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it an invaluable asset for various research endeavors.OAS2 is a vital enzyme involved in the innate immune response against viral infections, particularly in the activation of the antiviral endoribonuclease RNase L pathway.
Dysregulation of OAS2 has been implicated in various autoimmune diseases and viral infections, highlighting its importance as a potential therapeutic target and diagnostic marker.With the Human 2'5'-Oligoadenylate Synthetase 2 (OAS2) ELISA Kit, researchers can delve deeper into the intricate mechanisms governing the immune response, paving the way for novel discoveries and advancements in the fields of virology, immunology, and autoimmune disorders.
Product Name:
Human 2',5'-Oligoadenylate Synthetase 2 (OAS2) ELISA Kit
Product Code:
HUDL02123
Size:
96 Assays
Target:
OAS2
Synonyms:
p69 OAS / p71 OAS
Detection Method:
Sandwich
Range:
3.125-200ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
