The Human α2-M (Alpha-2 Macroglobulin) ELISA Kit is specifically designed to quantitatively measure the levels of α2-M in a variety of biological samples. Alpha-2 Macroglobulin, known for its broad protease inhibitor activity, plays a crucial role in inhibiting proteolytic enzymes in the body. This multifunctional protein acts as a sensor for protease activity and is involved in various physiological functions, including the acute phase response, immunity, and tissue remodeling processes. Accurate measurement of α2-M levels provides valuable insights into its role in modulating protease activity and immune responses, making it essential for understanding inflammatory conditions, infectious diseases, and tissue repair mechanisms.
The Human α2-M ELISA Kit from Assay Genie offers outstanding sensitivity and specificity, ensuring precise and reproducible results. With stringent quality control measures in place, this kit delivers reliable performance and user-friendly protocols, making it an excellent choice for both research and clinical applications. Researchers can trust Assay Genie's α2-M ELISA Kit for accurate and dependable quantification of this vital biomarker in their studies.
Product Name:
Human alpha2-M (Alpha-2 Macroglobulin) ELISA Kit
SKU:
AEES00096
Target:
Human α2-M (Alpha-2 Macroglobulin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 ng/mL
Detection range:
62.50-4000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human α2-M. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human α2-M and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human α2-M, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human α2-M. You can calculate the concentration of Human α2-M in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-93
99-109
82-95
Average (%)
90
105
90
1:4
Range (%)
88-100
87-101
88-98
Average (%)
96
94
95
1:8
Range (%)
93-103
84-96
93-105
Average (%)
97
91
100
1:16
Range (%)
88-104
85-98
91-98
Average (%)
96
90
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-112
105
EDTA plasma (n=5)
90-105
98
Cell culture media (n=5)
101-108
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
168.88
485.91
1661.69
163.4
475.24
1927.63
Standard deviation
9
23.47
75.11
8.2
21.96
87.71
C V (%)
5.33
4.83
4.52
5.02
4.62
4.55
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human α2-M concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human α2-M in samples. No significant cross-reactivity or interference between Human α2-M and analogues was observed.
