The Human 5'-NT (5'-nucleotidase) CLIA Kit is a powerful tool for detecting and quantifying levels of 5'-nucleotidase in human samples. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for research applications.5'-nucleotidase is an important enzyme involved in nucleotide metabolism, playing a key role in processes such as purine salvage and cell signaling. Dysregulation of 5'-nucleotidase has been implicated in various diseases, including liver diseases, cancer, and autoimmune disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
The Human 5'-NT CLIA Kit from AssayGenie provides researchers with a reliable tool for investigating the role of 5'-nucleotidase in health and disease. With its high performance and ease of use, this kit is an essential resource for advancing our understanding of nucleotide metabolism and its implications for human health.
Product Name:
Human 5-NT (5-Nucleotidase) CLIA Kit
SKU:
HUES00180
Target:
Human 5-NT (5-Nucleotidase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human 5-NT. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human 5-NT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human 5-NT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human 5-NT. You can calculate the concentration of Human 5-NT in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-98
86-98
85-97
Average (%)
93
92
91
1:4
Range (%)
92-106
101-114
100-120
Average (%)
100
107
109
1:8
Range (%)
96-110
83-95
83-99
Average (%)
103
90
90
1:16
Range (%)
87-102
85-96
92-104
Average (%)
94
91
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-103
95
EDTA plasma (n=5)
92-107
98
Cell culture media (n=5)
85-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
199.21
595.7
1640.79
210.42
621.13
1523.54
Standard deviation
16.26
48.55
179.99
18.29
52.73
155.4
C V (%)
8.16
8.15
10.97
8.69
8.49
10.2
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human 5-NT concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human 5-NT in samples. No significant cross-reactivity or interference between Human 5-NT and analogues was observed.
