Human A beta1-40 (Amyloid Beta Peptide 1-40) ELISA Kit
The Human Aβ1-40 (Amyloid Beta Peptide 1-40) ELISA Kit is specifically designed for the precise detection of levels of Aβ1-40 in human serum, plasma, and cell culture supernatants. This cutting-edge kit offers unparalleled sensitivity and specificity, ensuring accurate and reproducible results essential for a wide range of research applications.Aβ1-40 is a key protein involved in the pathogenesis of Alzheimer's disease, playing a crucial role in the formation of amyloid plaques in the brain. By accurately measuring Aβ1-40 levels, researchers can gain valuable insights into the progression of Alzheimer's and other neurodegenerative disorders, paving the way for the development of novel therapeutic strategies.
With its advanced technology and superior performance, the Human Aβ1-40 ELISA Kit is an indispensable tool for researchers looking to deepen their understanding of Alzheimer's disease and advance the field of neurology. Trust in the reliability and accuracy of this kit to drive groundbreaking discoveries and improve patient outcomes in the fight against Alzheimer's and related disorders.
Product Name:
Human A beta1-40 (Amyloid Beta Peptide 1-40) ELISA Kit
SKU:
HUES01733
Target:
Human A beta1-40 (Amyloid Beta Peptide 1-40)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Aβ1-40. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Aβ1-40 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Aβ1-40, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Aβ1-40. You can calculate the concentration of Human Aβ1-40 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
96-110
90-106
Average (%)
93
104
97
1:4
Range (%)
99-116
80-92
95-111
Average (%)
106
87
102
1:8
Range (%)
100-113
84-98
96-108
Average (%)
106
90
102
1:16
Range (%)
97-110
81-92
97-109
Average (%)
105
87
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
101
EDTA plasma (n=5)
85-96
91
Cell culture media (n=5)
92-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.6
152.1
454.7
45.8
156.8
436.8
Standard deviation
3.3
6.1
23.6
2.6
9.1
13.1
C V (%)
6.52
4.01
5.19
5.68
5.8
3.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human Aβ1-40 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Aβ1-40 in samples. No significant cross-reactivity or interference between Human Aβ1-40 and analogues was observed.
