Human A Disintegrin And Metalloprotease 15 (ADAM15) ELISA Kit
The Human A Disintegrin and Metalloprotease 15 (ADAM15) ELISA Kit is a powerful tool for detecting and quantifying ADAM15 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it a valuable asset for a wide range of research applications.ADAM15 is a key enzyme involved in various cellular processes, including cell adhesion, migration, and signaling.
Dysregulation of ADAM15 has been linked to inflammatory diseases, cancer, and immune disorders, highlighting its importance as a potential biomarker and therapeutic target.By using the Human ADAM15 ELISA Kit, researchers can gain valuable insights into the role of ADAM15 in disease pathogenesis and progression, ultimately paving the way for the development of novel treatment strategies. Trust in this kit to deliver reliable data and support your research endeavors.
Product Name:
Human A Disintegrin And Metalloprotease 15 (ADAM15) ELISA Kit
Product Code:
HUDL00058
Size:
96 Assays
Target:
ADAM15
Synonyms:
MDC15, Metargidin, Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15, Metalloprotease RGD disintegrin protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
