Human A Disintegrin And Metalloprotease 22 (ADAM22) ELISA Kit
The Human A Disintegrin and Metalloprotease 22 (ADAM22) ELISA Kit is a powerful tool for detecting levels of ADAM22 in human samples with high accuracy. This kit is suitable for use with serum, plasma, and cell culture supernatants, providing reliable and reproducible results for a variety of research applications.ADAM22 is a key enzyme involved in cell adhesion and migration, making it a crucial player in processes such as cell signaling and development. Dysregulation of ADAM22 has been implicated in various diseases, including cancer and neurological disorders.
Therefore, measuring ADAM22 levels can provide important insights into disease progression and potential treatment strategies.With its high sensitivity and specificity, the Human ADAM22 ELISA Kit offers researchers a valuable tool for studying the role of ADAM22 in health and disease. Its easy-to-use format and accurate results make it a must-have for any laboratory conducting research in this field.
Product Name:
Human A Disintegrin And Metalloprotease 22 (ADAM22) ELISA Kit
Product Code:
HUDL00061
Size:
96 Assays
Target:
ADAM22
Synonyms:
MDC2, Disintegrin And Metalloproteinase Domain-Containing Protein 22, Metalloproteinase-Like,Disintegrin-Like,And Cysteine-Rich Protein 2
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
