SynonymsADAM23,MDCL,eMDCII,Metalloproteinase-like, disintegrin-like, and cysteine-rich protein L,Epididymal metalloproteinase-like, disintegrin-like, and cysteine-rich protein II
ReactivityHuman
Sample TypeSerum, Plasma, Tissue Homogenates And Other Biological Fluids
SKU:
HUDL00062
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9UKQ2
Sensitivity:
0.061ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
ADAM23,MDCL,eMDCII,Metalloproteinase-like, disintegrin-like, and cysteine-rich protein L,Epididymal metalloproteinase-like, disintegrin-like, and cysteine-rich protein II
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Human A Disintegrin And Metalloprotease 28 (ADAM28) ELISA Kit
The Human A Disintegrin and Metalloprotease 28 (ADAM28) ELISA Kit is a highly sensitive and specific assay designed for the detection of ADAM28 levels in human serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it ideal for a wide range of research applications.ADAM28 is a protein that belongs to the ADAM (a disintegrin and metalloprotease) family, known for its role in cell adhesion and proteolysis. It is involved in various physiological and pathological processes, including inflammation, tissue remodeling, and cancer progression.
By measuring ADAM28 levels, researchers can gain valuable insights into these processes and potentially identify new therapeutic targets.Overall, the Human ADAM28 ELISA Kit is a valuable tool for studying the role of ADAM28 in various diseases and biological processes, providing researchers with the means to advance their understanding and potentially develop new treatment strategies.
Product Name:
Human A Disintegrin And Metalloprotease 28 (ADAM28) ELISA Kit
Product Code:
HUDL00062
Size:
96 Assays
Target:
ADAM28
Synonyms:
ADAM23, MDCL, eMDCII, Metalloproteinase-like, disintegrin-like, and cysteine-rich protein L, Epididymal metalloproteinase-like, disintegrin-like, and cysteine-rich protein II
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
