Human A Disintegrin And Metalloprotease 33 (ADAM33) ELISA Kit
The Human ADAM33 ELISA Kit is a reliable tool for detecting levels of A Disintegrin and Metalloprotease 33 (ADAM33) in human serum, plasma, and cell culture supernatants. This highly sensitive and specific kit provides accurate and reproducible results, making it perfect for a variety of research applications.ADAM33 is an important enzyme involved in cell communication and tissue remodeling, with implications in diseases such as asthma, chronic obstructive pulmonary disease (COPD), and other respiratory conditions.
Understanding ADAM33 levels can provide valuable insights into disease progression and potential treatment options.By enabling researchers to measure ADAM33 levels with precision, the Human ADAM33 ELISA Kit facilitates the study of this enzyme's role in various diseases and may contribute to the development of new therapeutic strategies.
Product Name:
Human A Disintegrin And Metalloprotease 33 (ADAM33) ELISA Kit
Product Code:
HUDL00063
Size:
96 Assays
Target:
ADAM33
Synonyms:
C20orf153, Disintegrin and metalloproteinase domain-containing protein 33
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
