Human ABCA1 (ATP-binding cassette transporter A1) CLIA Kit
The Human ABCA1 (ATP-binding cassette transporter A1) CLIA Kit is a highly reliable and accurate tool for measuring levels of ABCA1 in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.ABCA1 is a vital protein involved in lipid transport and cholesterol homeostasis, playing a crucial role in conditions such as cardiovascular diseases, Alzheimer's disease, and atherosclerosis.
By accurately measuring ABCA1 levels, researchers can gain valuable insights into these conditions and potentially develop targeted therapies.Overall, the Human ABCA1 CLIA Kit is an essential tool for studying the role of ABCA1 in health and disease, providing researchers with the tools they need to make significant advancements in the field of lipid biology and cardiovascular research.
Product Name:
Human ABCA1 (ATP-binding cassette transporter A1) CLIA Kit
SKU:
HUES00371
Target:
Human ABCA1 (ATP-binding cassette transporter A1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ABCA1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ABCA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ABCA1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ABCA1. You can calculate the concentration of Human ABCA1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-103
93-111
101-119
Average (%)
97
101
109
1:4
Range (%)
100-114
97-109
91-105
Average (%)
106
103
98
1:8
Range (%)
89-102
88-99
97-110
Average (%)
95
94
104
1:16
Range (%)
92-104
90-102
90-101
Average (%)
98
97
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-116
106
EDTA plasma (n=5)
94-106
100
Cell culture media (n=5)
87-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.55
293.75
912.59
99.94
276.53
980.13
Standard deviation
11.76
31.49
100.75
11.55
32.11
79.39
C V (%)
12.31
10.72
11.04
11.56
11.61
8.1
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ABCA1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ABCA1 in samples. No significant cross-reactivity or interference between Human ABCA1 and analogues was observed.
