Human ABCG1 (ATP Binding Cassette Transporter G1) CLIA Kit
The Human ABCG1 (ATP Binding Cassette Transporter G1) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of ABCG1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a wide range of research applications.ABCG1 is an important protein involved in the transport of various molecules across cell membranes, playing a critical role in lipid metabolism and cholesterol homeostasis. Dysregulation of ABCG1 has been linked to a variety of diseases, including cardiovascular diseases, Alzheimer's disease, and atherosclerosis, making it a valuable biomarker for studying these conditions and developing potential therapies.
With its high sensitivity and specificity, the Human ABCG1 CLIA Kit offers researchers a valuable tool for studying the role of ABCG1 in disease pathogenesis and exploring potential therapeutic targets. Trust in the accuracy and reliability of this kit for all your research needs.
Product Name:
Human ABCG1 (ATP Binding Cassette Transporter G1) CLIA Kit
SKU:
HUES00370
Target:
Human ABCG1 (ATP Binding Cassette Transporter G1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ABCG1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ABCG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ABCG1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ABCG1. You can calculate the concentration of Human ABCG1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
93-108
89-105
Average (%)
96
99
96
1:4
Range (%)
91-106
98-113
96-114
Average (%)
97
105
104
1:8
Range (%)
98-114
89-100
86-101
Average (%)
104
94
93
1:16
Range (%)
91-103
94-110
94-107
Average (%)
96
102
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
94
EDTA plasma (n=5)
87-101
93
Cell culture media (n=5)
85-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.36
258.81
673.22
101.87
238.81
618.16
Standard deviation
8.56
25.91
50.76
12.47
27.1
43.46
C V (%)
8.7
10.01
7.54
12.24
11.35
7.03
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ABCG1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ABCG1 in samples. No significant cross-reactivity or interference between Human ABCG1 and analogues was observed.
