Human ACE2 (Angiotensin I Converting Enzyme 2) CLIA Kit
Based on the information provided in the URL, here is a product description: The Human ACE2 (Angiotensin I Converting Enzyme 2) CLIA Kit is a cutting-edge diagnostic tool designed for the precise measurement of ACE2 levels in human samples such as serum, plasma, and cell culture supernatants. This advanced kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results, which makes it an invaluable asset for various research applications. ACE2 is a key enzyme involved in the Renin-Angiotensin System, playing a crucial role in regulating blood pressure and cardiovascular function.
Additionally, ACE2 is the cellular receptor for the entry of SARS-CoV-2, the virus responsible for COVID-19, making it a vital target for understanding viral infections and developing therapeutic interventions. By utilizing the Human ACE2 CLIA Kit, researchers can gain valuable insights into the role of ACE2 in cardiovascular diseases, respiratory infections, and other health conditions. This kit serves as an essential tool for studying the mechanisms of disease progression and exploring potential treatments, ultimately contributing to advancements in medical research and patient care.
Product Name:
Human ACE2 (Angiotensin I Converting Enzyme 2) CLIA Kit
SKU:
HUES00209
Target:
Human ACE2 (Angiotensin I Converting Enzyme 2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ACE2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACE2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACE2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ACE2. You can calculate the concentration of Human ACE2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-103
87-101
90-105
Average (%)
98
93
97
1:4
Range (%)
100-114
85-99
101-116
Average (%)
106
92
108
1:8
Range (%)
96-108
92-104
98-116
Average (%)
102
98
106
1:16
Range (%)
98-112
88-103
98-115
Average (%)
106
94
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-114
108
EDTA plasma (n=5)
86-100
92
Cell culture media (n=5)
89-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
264.48
543.75
1692.47
286.76
519.37
1672.55
Standard deviation
23.7
59.21
149.95
31.08
37.13
192.18
C V (%)
8.96
10.89
8.86
10.84
7.15
11.49
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ACE2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ACE2 in samples. No significant cross-reactivity or interference between Human ACE2 and analogues was observed.
