The Human ACO1 (Aconitase 1) CLIA Kit is specifically designed for the accurate measurement of ACO1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research purposes.ACO1, also known as aconitase 1, is a key enzyme in the citric acid cycle and plays a crucial role in cellular energy production.
Dysregulation of ACO1 has been linked to various diseases, including metabolic disorders and neurodegenerative conditions.By detecting and measuring ACO1 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets. The Human ACO1 CLIA Kit provides a reliable tool for studying ACO1 function and its implications in various pathological conditions.
Product Name:
Human ACO1 (Aconitase 1) CLIA Kit
SKU:
HUES00249
Target:
Human ACO1 (Aconitase 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ACO1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACO1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACO1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ACO1. You can calculate the concentration of Human ACO1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
100-118
89-101
Average (%)
96
107
96
1:4
Range (%)
99-116
86-99
103-118
Average (%)
106
91
109
1:8
Range (%)
86-99
87-100
86-96
Average (%)
92
93
91
1:16
Range (%)
103-117
99-116
97-113
Average (%)
109
106
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
100
EDTA plasma (n=5)
98-110
104
Cell culture media (n=5)
92-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.87
286.48
886.35
115.85
276.99
826.38
Standard deviation
11.42
28.39
70.64
12.91
25.46
50.24
C V (%)
10.69
9.91
7.97
11.14
9.19
6.08
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ACO1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ACO1 in samples. No significant cross-reactivity or interference between Human ACO1 and analogues was observed.
