Human ACP5 (Tartrate Resistant Acid Phosphatase 5) CLIA Kit
The Human ACP5 (Tartrate-Resistant Acid Phosphatase 5) CLIA Kit is specially designed for the accurate and precise measurement of ACP5 levels in human samples, including serum, plasma, and cell culture supernatants. This kit delivers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.ACP5 is an important enzyme involved in bone resorption and remodeling, playing a critical role in diseases like osteoporosis and Paget's disease.
By accurately measuring ACP5 levels, researchers can gain valuable insights into bone metabolism and potential therapeutic strategies for these conditions.Overall, the Human ACP5 CLIA Kit offers researchers a powerful tool for studying the role of ACP5 in bone health and disease, providing valuable information for advancing our understanding of bone-related disorders and developing targeted interventions.
Product Name:
Human ACP5 (Tartrate Resistant Acid Phosphatase 5) CLIA Kit
SKU:
HUES00879
Target:
Human ACP5 (Tartrate Resistant Acid Phosphatase 5)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ACP5. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACP5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACP5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ACP5. You can calculate the concentration of Human ACP5 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-101
100-113
89-103
Average (%)
96
106
95
1:4
Range (%)
99-115
101-116
94-105
Average (%)
106
107
99
1:8
Range (%)
88-102
90-105
96-108
Average (%)
95
96
103
1:16
Range (%)
100-113
88-101
91-102
Average (%)
106
94
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-106
98
EDTA plasma (n=5)
90-105
96
Cell culture media (n=5)
89-102
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.55
0.91
4.72
0.56
0.97
4.61
Standard deviation
0.07
0.1
0.51
0.06
0.11
0.39
C V (%)
12.73
10.99
10.81
10.71
11.34
8.46
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ACP5 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ACP5 in samples. No significant cross-reactivity or interference between Human ACP5 and analogues was observed.
