Human ACP5 (Tartrate Resistant Acid Phosphatase 5) ELISA Kit
The Human ACP5 (Tartrate-Resistant Acid Phosphatase 5) ELISA Kit is specifically designed for the accurate and efficient detection of ACP5 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and reproducible results for a variety of research purposes.ACP5, also known as tartrate-resistant acid phosphatase 5, is an important enzyme involved in bone metabolism and osteoclast function.
It plays a crucial role in bone resorption and remodeling processes, making it a key biomarker for studying bone-related diseases such as osteoporosis and osteopetrosis.With its reliable performance and ease of use, the Human ACP5 ELISA Kit is a valuable tool for researchers studying bone biology, bone disorders, and potential therapeutic interventions targeting the regulation of ACP5 levels.
Product Name:
Human ACP5 (Tartrate Resistant Acid Phosphatase 5) ELISA Kit
SKU:
HUES02565
Target:
Human ACP5 (Tartrate Resistant Acid Phosphatase 5)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TRACP-5b. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TRACP-5b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TRACP-5b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TRACP-5b. You can calculate the concentration of Human TRACP-5b in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
91-106
89-104
Average (%)
98
97
96
1:4
Range (%)
92-105
86-100
88-100
Average (%)
98
91
94
1:8
Range (%)
87-98
81-92
88-99
Average (%)
93
87
94
1:16
Range (%)
86-99
86-98
87-102
Average (%)
93
92
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-101
93
EDTA plasma (n=5)
89-102
95
Cell culture media (n=5)
86-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.44
6.89
18.97
2.55
6.23
18.31
Standard deviation
0.16
0.34
0.64
0.13
0.32
0.62
C V (%)
6.56
4.93
3.37
5.1
5.14
3.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TRACP-5b concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TRACP-5b in samples. No significant cross-reactivity or interference between Human TRACP-5b and analogues was observed.
