Description
Human ACTalpha2 (Actin Alpha 2, Smooth Muscle) ELISA Kit
The ACT?2 ELISA Kit is expertly designed for the quantitative detection of Actin Alpha 2, Smooth Muscle (ACT?2) levels in various human biological samples.
ACT?2 is a significant protein involved in various cellular activities, such as cell motility, structure, and integrity. It plays a crucial role in smooth muscle contraction and has been linked with various muscular and cell motility disorders. Accurate measurement of ACT?2 is vital for understanding its role in disease progression and for the development of targeted therapeutic strategies. Assay Genie's ACT?2 ELISA Kit provides exceptional sensitivity and specificity, ensuring reliable, and reproducible results. Manufactured adhering to strict quality control standards, this kit delivers robust performance and is user-friendly, making it an excellent choice for both research and clinical applications. Trust in Assay Genie's ACT?2 ELISA Kit to provide accurate and reliable quantification of this critical biomarker in your studies.
| Product Name: | Human ACTalpha2 (Actin Alpha 2, Smooth Muscle) ELISA Kit |
| Product Code: | AEES00030 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Human |
| Detection Range: | 3.13-200 ng/mL |
| Sensitivity: | 1.88 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Human ACT?2 in samples. No significant cross-reactivity or interference between Human ACT?2 and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Human ACT?2 concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ACT?2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACT?2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACT?2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ACT?2. You can calculate the concentration of Human ACT?2 in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
|
| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
