Human Activity Regulated Cytoskeleton Associated Protein (ARC) ELISA Kit
The Human Activity-Regulated Cytoskeleton-Associated Protein (ARC) ELISA Kit is specifically designed for the accurate measurement of ARC levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reliable results, making it suitable for a variety of research applications.ARC is a critical protein that plays a key role in regulating cytoskeletal dynamics and synaptic plasticity in the brain. It is known to be involved in processes such as memory formation, learning, and neuronal development.
Abnormalities in ARC expression have been linked to various neurological disorders, making it a valuable biomarker for studying these conditions and potentially identifying new therapeutic targets.Overall, the ARC ELISA Kit provides researchers with a valuable tool for studying the role of ARC in neuronal function and exploring its potential implications in neurological disorders.
Product Name:
Human Activity Regulated Cytoskeleton Associated Protein (ARC) ELISA Kit
Product Code:
HUDL00235
Size:
96 Assays
Target:
ARC
Synonyms:
Arg3.1, Activity-regulated gene 3.1 protein homolog
Detection Method:
Sandwich
Range:
78.125-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
