The Human Adropin ELISA Kit is a cutting-edge assay designed for the precise measurement of adropin levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it ideal for various research applications.Adropin is a critical protein involved in regulating energy metabolism and lipid metabolism, playing a key role in conditions like obesity, diabetes, and cardiovascular diseases.
By detecting adropin levels, researchers can gain valuable insights into these conditions and potentially develop targeted therapies for better patient outcomes.Overall, the Human Adropin ELISA Kit is a valuable tool for advancing research in the fields of metabolism, obesity, and cardiovascular health, offering researchers a reliable method for studying adropin and its impact on human health.
Product Name:
Human AD (Adropin) ELISA kit
SKU:
HUES03400
Target:
Human AD (Adropin) ELISA kit
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AD. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AD, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human AD. You can calculate the concentration of Human AD in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
97-108
83-96
Average (%)
98
103
90
1:4
Range (%)
86-99
84-95
89-102
Average (%)
93
90
94
1:8
Range (%)
88-98
82-93
83-95
Average (%)
93
88
89
1:16
Range (%)
86-98
86-100
85-96
Average (%)
93
91
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-104
97
EDTA plasma (n=5)
89-102
95
Cell culture media (n=5)
89-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
43.68
95.22
350.77
41.0
89.88
330.27
Standard deviation
2.97
3.93
16.77
2.85
3.79
14.47
C V (%)
6.8
4.13
4.78
6.95
4.22
4.38
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human AD concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AD in samples. No significant cross-reactivity or interference between Human AD and analogues was observed.
