Human ADAMTS5 (A Disintegrin And Metalloproteinase With Thrombospondin 5) ELISA Kit
The Human ADAMTS5 (A Disintegrin and Metalloproteinase with Thrombospondin 5) ELISA Kit is a powerful tool designed for the accurate measurement of ADAMTS5 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research purposes.ADAMTS5 is a key enzyme involved in extracellular matrix degradation and tissue remodeling processes. Dysregulation of ADAMTS5 has been linked to various diseases, including arthritis, cardiovascular diseases, and cancer.
By accurately quantifying ADAMTS5 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.Overall, the Human ADAMTS5 ELISA Kit is a valuable asset for researchers studying the role of ADAMTS5 in health and disease, offering precise and reliable measurement of this important enzyme in human samples.
Product Name:
Human ADAMTS5 (A Disintegrin And Metalloproteinase With Thrombospondin 5) ELISA Kit
SKU:
HUES03562
Target:
Human ADAMTS5 (A Disintegrin And Metalloproteinase With Thrombospondin 5)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ADAMTS5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADAMTS5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADAMTS5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ADAMTS5. You can calculate the concentration of Human ADAMTS5 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-103
88-98
86-102
Average (%)
94
93
93
1:4
Range (%)
86-98
81-92
86-96
Average (%)
92
86
91
1:8
Range (%)
93-106
84-96
87-100
Average (%)
100
91
92
1:16
Range (%)
87-102
85-98
83-94
Average (%)
94
90
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-110
102
EDTA plasma (n=5)
92-104
98
Cell culture media (n=5)
86-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.57
7.27
23.18
2.57
7.13
24.01
Standard deviation
0.17
0.32
0.99
0.16
0.41
0.79
C V (%)
6.61
4.4
4.27
6.23
5.75
3.29
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ADAMTS5 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ADAMTS5 in samples. No significant cross-reactivity or interference between Human ADAMTS5 and analogues was observed.
