Human Adaptor Related Protein Complex 2 Mu 1 (AP2m1) ELISA Kit
The Human Adaptor-Related Protein Complex 2 Mu 1 (AP2M1) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of AP2M1 levels in human serum, plasma, and cell culture supernatants. This kit provides robust and reliable results, making it an invaluable tool for a variety of research applications.AP2M1 is a key component of the adaptor protein complex 2 (AP2), which plays a critical role in clathrin-mediated endocytosis and intracellular trafficking. Dysregulation of AP2M1 has been implicated in various diseases, including neurodegenerative disorders and cancer.
Therefore, the measurement of AP2M1 levels can provide valuable insights into disease mechanisms and potential therapeutic targets.With its high sensitivity and specificity, the Human AP2M1 ELISA Kit offers researchers a powerful tool for studying the role of AP2M1 in health and disease. Its ease of use and reproducibility make it suitable for a wide range of experimental settings, making it a valuable addition to any research laboratory.
Product Name:
Human Adaptor Related Protein Complex 2 Mu 1 (AP2m1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
88-100
94
EDTA plasma (n=5)
90-101
95
Heparin plasma (n=5)
92-101
96
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
