Human Adhesion Molecule With Ig Like Domain Protein 1 (AMIGO1) ELISA Kit
The Human AMIGO1 (Adhesion Molecule with Ig-like Domain Protein 1) ELISA Kit is specifically designed for the precise detection of AMIGO1 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity to ensure accurate and reproducible results, making it an invaluable tool for a variety of research studies. AMIGO1 is a cell adhesion molecule that contains an Ig-like domain and is involved in cell-cell adhesion and signaling processes.
It plays a critical role in neuronal development, synaptic plasticity, and cell migration, making it a key molecule in neurological disorders, cancer metastasis, and other pathological conditions. The AMIGO1 ELISA Kit provides researchers with a reliable method for quantifying AMIGO1 levels, allowing for a better understanding of its functions and potential therapeutic implications.
Product Name:
Human Adhesion Molecule With Ig Like Domain Protein 1 (AMIGO1) ELISA Kit
Product Code:
HUDL00157
Size:
96 Assays
Target:
AMIGO1
Synonyms:
ALI2, Alivin-2, Amphoterin-Induced Gene And Open Reading Frame, Amphoterin-induced protein 1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
