The Human Adipor1 (Adiponectin Receptor 1) CLIA Kit is specifically designed for the accurate detection of Adiponectin Receptor 1 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures reliable and reproducible results, making it an ideal tool for a wide range of research applications related to adiponectin signaling pathways.Adiponectin Receptor 1 is a key receptor involved in mediating the effects of adiponectin, a hormone that plays a crucial role in regulating metabolism and insulin sensitivity. Dysregulation of adiponectin signaling is associated with various metabolic disorders, such as obesity, type 2 diabetes, and cardiovascular diseases, making Adiponectin Receptor 1 a valuable biomarker for studying these conditions and developing potential therapeutic strategies.
Overall, the Human Adipor1 CLIA Kit provides researchers with a reliable and efficient method for studying the role of Adiponectin Receptor 1 in metabolic disorders and other related conditions, ultimately contributing to the advancement of medical research and the development of targeted therapies.
Product Name:
Human ADIPOR1 (Adiponectin Receptor 1) CLIA Kit
SKU:
HUES00202
Target:
Human ADIPOR1 (Adiponectin Receptor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ADIPOR1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADIPOR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADIPOR1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ADIPOR1. You can calculate the concentration of Human ADIPOR1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
88-98
96-110
Average (%)
99
93
102
1:4
Range (%)
96-108
98-114
89-106
Average (%)
101
104
96
1:8
Range (%)
90-102
96-110
84-100
Average (%)
95
103
91
1:16
Range (%)
98-113
91-107
98-115
Average (%)
104
99
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-106
99
EDTA plasma (n=5)
83-98
90
Cell culture media (n=5)
91-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.4
3.67
0.46
1.35
3.47
Standard deviation
0.05
0.11
0.38
0.06
0.12
0.25
C V (%)
10.0
7.86
10.35
13.04
8.89
7.2
Sample type &Sample volume:
Tissue homogenates,cell lysates and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ADIPOR1 concentrations in Tissue homogenates,cell lysates and other biological fluids.
Specificity:
This kit recognizes Human ADIPOR1 in samples. No significant cross-reactivity or interference between Human ADIPOR1 and analogues was observed.
