Human Adrenergic Receptor Beta 2 (ADRb2) ELISA Kit
The Human Adrenergic Receptor Beta-2 (ADRB2) ELISA Kit is specifically designed for the precise detection of ADRB2 levels in human serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers consistent and dependable results, making it an excellent choice for a variety of research applications.ADRB2 is a key protein involved in regulating the body's response to stress and is a crucial component in the sympathetic nervous system.
This receptor plays a vital role in conditions such as asthma, heart failure, and obesity, making it an important target for studying these diseases and developing potential treatments.By using the Human ADRB2 ELISA Kit, researchers can accurately measure ADRB2 levels, gaining valuable insights into its function and potential therapeutic implications. This kit is an essential tool for researchers working in the fields of pharmacology, cardiology, and respiratory medicine.
Product Name:
Human Adrenergic Receptor Beta 2 (ADRb2) ELISA Kit
Product Code:
HUDL00094
Size:
96 Assays
Synonyms:
B2AR, Beta-2 adrenoreceptor
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-99
91
EDTA plasma (n=5)
80-99
89
Heparin plasma (n=5)
82-99
90
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
