Human ADRP (Adipose Differentiation Related Protein) CLIA Kit
The Human ADRP (Adipose Differentiation-Related Protein) CLIA Kit is specifically designed for the precise measurement of ADRP levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.ADRP is a key protein involved in adipocyte differentiation and lipid metabolism, making it a valuable biomarker in the study of metabolic disorders, obesity, and related conditions.
By accurately measuring ADRP levels, researchers can gain insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets for intervention. Invest in the Human ADRP CLIA Kit for reliable and reproducible data that will advance your research in the fields of metabolism, obesity, and beyond.
Product Name:
Human ADRP (Adipose Differentiation Related Protein) CLIA Kit
SKU:
HUES00206
Target:
Human ADRP (Adipose Differentiation Related Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ADRP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADRP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ADRP. You can calculate the concentration of Human ADRP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-103
99-113
99-112
Average (%)
97
105
106
1:4
Range (%)
95-109
100-118
88-103
Average (%)
102
108
96
1:8
Range (%)
86-99
95-106
87-98
Average (%)
93
101
93
1:16
Range (%)
84-99
86-100
101-117
Average (%)
91
92
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-101
95
EDTA plasma (n=5)
100-114
107
Cell culture media (n=5)
101-115
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.53
176.41
777.37
92.46
188.34
841.89
Standard deviation
8.82
13.88
69.34
10.56
15.73
53.12
C V (%)
8.77
7.87
8.92
11.42
8.35
6.31
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ADRP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ADRP in samples. No significant cross-reactivity or interference between Human ADRP and analogues was observed.
