The Human AE-binding Protein-1 (AEBP1) ELISA Kit is a reliable and accurate tool for detecting AEBP1 levels in human samples including serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this ELISA kit delivers consistent and reproducible results, making it ideal for various research applications.AEBP1 is a key protein involved in cellular functions such as cell growth, adhesion, and inflammation. Its dysregulation has been linked to various diseases including cancer, cardiovascular disorders, and metabolic syndromes.
By accurately measuring AEBP1 levels, researchers can gain valuable insights into the pathophysiology of these conditions and potentially identify novel therapeutic targets.Overall, the Human AE-binding Protein-1 (AEBP1) ELISA Kit offers a powerful tool for studying the role of AEBP1 in human health and disease, providing researchers with the necessary tools to advance their research in a diverse range of fields.
Product Name:
Human AE Binding Protein 1 (AEBP1) ELISA Kit
Product Code:
HUDL00098
Size:
96 Assays
Target:
AEBP1
Synonyms:
ACLP, Aortic Carboxypeptidase-Like Protein, Adipocyte Enhancer Binding Protein 1
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
