Human AGGF1 (Angiogenic Factor with G Patch and FHA Domains 1) CLIA Kit
The Human AGGF1 (Angiogenic Factor with G patch and FHA domains 1) CLIA Kit is designed for the precise measurement of AGGF1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and consistent results, making it suitable for various research applications.
AGGF1 is a key protein with roles in promoting angiogenesis and influencing cell growth. Its involvement in conditions like cancer, cardiovascular diseases, and neurodegenerative disorders makes it a valuable biomarker for investigating these diseases and developing possible treatments.
Product Name:
Human AGGF1 (Angiogenic Factor with G Patch and FHA Domains 1) CLIA Kit
SKU:
HUES00162
Target:
Human AGGF1 (Angiogenic Factor with G Patch and FHA Domains 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AGGF1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AGGF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AGGF1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AGGF1. You can calculate the concentration of Human AGGF1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
87-101
96-112
Average (%)
103
94
102
1:4
Range (%)
84-95
100-112
89-103
Average (%)
90
105
95
1:8
Range (%)
93-108
89-104
98-110
Average (%)
101
95
104
1:16
Range (%)
99-115
95-111
100-115
Average (%)
105
101
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
95
EDTA plasma (n=5)
95-107
102
Cell culture media (n=5)
97-113
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.42
208.77
827.42
113.14
209.48
818.85
Standard deviation
12.57
16.97
75.96
13.36
14.79
73.7
C V (%)
11.7
8.13
9.18
11.81
7.06
9.0
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human AGGF1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AGGF1 in samples. No significant cross-reactivity or interference between Human AGGF1 and analogues was observed.
