The Human AGR2 (Anterior Gradient Protein 2) CLIA Kit is a powerful tool for the quantitative measurement of AGR2 levels in human samples. This kit offers exceptional sensitivity and accuracy, allowing researchers to obtain reliable and reproducible data for their studies.AGR2 is a vital protein that has been linked to various diseases, including cancer and inflammatory conditions. By measuring AGR2 levels, researchers can gain valuable insights into disease progression, prognosis, and potential treatment strategies.
Whether you are investigating the role of AGR2 in cancer metastasis, exploring its involvement in inflammatory pathways, or studying its impact on cellular processes, the Human AGR2 CLIA Kit provides the precision and reliability needed for successful research outcomes. Order your kit today and unlock the potential of AGR2 biomarker analysis.
Product Name:
Human AGR2 (Anterior Gradient Protein 2) CLIA Kit
SKU:
HUES00218
Target:
Human AGR2 (Anterior Gradient Protein 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AGR2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AGR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AGR2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AGR2. You can calculate the concentration of Human AGR2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-116
100-114
86-101
Average (%)
108
106
93
1:4
Range (%)
95-109
87-103
89-102
Average (%)
100
94
95
1:8
Range (%)
102-115
99-114
88-103
Average (%)
109
105
96
1:16
Range (%)
87-97
99-114
85-99
Average (%)
92
106
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
94
EDTA plasma (n=5)
84-98
91
Cell culture media (n=5)
92-109
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.65
170.57
861.73
114.7
186.33
864.08
Standard deviation
9.96
19.31
63.34
12.15
19.88
57.55
C V (%)
9.0
11.32
7.35
10.59
10.67
6.66
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human AGR2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AGR2 in samples. No significant cross-reactivity or interference between Human AGR2 and analogues was observed.
